Cells, Free Full-Text

Viruses are one of the most important concerns for human health, and overcoming viral infections is a worldwide challenge. However, researchers have been trying to manipulate viral genomes to overcome various disorders, including cancer, for vaccine development purposes. CRISPR (clustered regularly interspaced short palindromic repeats) is becoming one of the most functional and widely used tools for RNA and DNA manipulation in multiple organisms. This approach has provided an unprecedented opportunity for creating simple, inexpensive, specific, targeted, accurate, and practical manipulations of viruses, such as severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), human immunodeficiency virus-1 (HIV-1), and vaccinia virus. Furthermore, this method can be used to make an effective and precise diagnosis of viral infections. Nevertheless, a valid and scientifically designed CRISPR system is critical to make more effective and accurate changes in viruses. In this review, we have focused on the best and the most effective ways to design sgRNA, gene knock-in(s), and gene knock-out(s) for virus-targeted manipulation. Furthermore, we have emphasized the application of CRISPR technology in virus diagnosis and in finding significant genes involved in virus-host interactions.

Iec/Ts 62886 Get File - Colaboratory

Cells, Free Full-Text

Cancer type classification using plasma cell-free RNAs derived

The dependence of cell-free protein synthesis in E. coli upon

Cell-Free DNA and Apoptosis: How Dead Cells Inform About the

The cell-free system: A new apparatus for affordable, sensitive

Cell-free DNA tissues of origin by methylation profiling reveals

Cancers, Free Full-Text

Polimer Program Get File - Colaboratory

Harnessing Extracellular Vesicles for Regenerative Therapy

Cancers, Free Full-Text