Exploring the role of monocyte chemoattractant protein-1 in fibroblast-like synovial cells in rheumatoid arthritis [PeerJ]
Por um escritor misterioso
Last updated 01 abril 2025
![Exploring the role of monocyte chemoattractant protein-1 in fibroblast-like synovial cells in rheumatoid arthritis [PeerJ]](https://dfzljdn9uc3pi.cloudfront.net/2021/11973/1/fig-5-full.png)
Background Rheumatoid arthritis (RA) is a chronic systemic inflammatory disease with persistent synovitis. In the present study, the impact of monocyte chemoattractant protein-1 (MCP-1) was explored to determine methods for the diagnosis and treatment of RA. Methods First, fibroblast-like synoviocytes (FLSs) were obtained from a collagen-induced rat RA model. Next, MCP-1-overexpression plasmid and small interfering RNA were transfected into human and rat FLSs. Cell Counting Kit-8 (CCK-8), Transwell migration and flow cytometry assays were used to analyze cell proliferation, migration and apoptosis of FLSs following MCP-1 transfections, respectively. Furthermore, western blotting was used to analyze the expression levels of p-P38, p-PI3K, PI3K, CD31, VEGF, TNF-α and IL-β in FLSs following MCP-1 transfection. In addition, reverse transcription-quantitative PCR and ELISAs were used to analyze the expression levels of C-reactive protein (CRP), estrogen receptor, MCP-1 and pentraxin-3 in patients with clinical RA, followed by correlation analysis of clinical data. Finally, expression validation, diagnostic and protein-protein interaction (PPI) network analysis of MCP-1 were performed. Results MCP-1 promoted FLS proliferation and migration, and affected the apoptosis of FLSs. In addition, the expression levels of p-P38, p-PI3K, PI3K, CD31, VEGF, TNF-α and IL-β were also affected by MCP-1. In patients with clinical RA, the expression level of MCP-1 was increased. Moreover, CRP expression level was significantly up-regulated in RA. Clinically, MCP-1 was strongly correlated with tender joint count, swollen joint count, visual analog scale for general health and disease activity score 28 (DAS28)-MCP-1, and was moderately correlated with DAS28 and DAS28-CRP. PPI analysis showed that MCP-1 mainly interacted with other inflammatory cytokines. Conclusion In conclusion, MCP-1 may play a significant regulatory role in RA, and could be used as a measurement index of clinical RA activity.
![Exploring the role of monocyte chemoattractant protein-1 in fibroblast-like synovial cells in rheumatoid arthritis [PeerJ]](https://ars.els-cdn.com/content/image/1-s2.0-S0753332222002359-gr6.jpg)
The enhanced mitochondrial dysfunction by cantleyoside confines inflammatory response and promotes apoptosis of human HFLS-RA cell line via AMPK/Sirt 1/NF-κB pathway activation - ScienceDirect
![Exploring the role of monocyte chemoattractant protein-1 in fibroblast-like synovial cells in rheumatoid arthritis [PeerJ]](https://media.springernature.com/m685/springer-static/image/art%3A10.1007%2Fs12015-023-10517-1/MediaObjects/12015_2023_10517_Fig2_HTML.png)
Conditioned Medium – Is it an Undervalued Lab Waste with the Potential for Osteoarthritis Management?
![Exploring the role of monocyte chemoattractant protein-1 in fibroblast-like synovial cells in rheumatoid arthritis [PeerJ]](https://www.medrxiv.org/content/medrxiv/early/2020/07/19/2020.07.16.20155291/F7.large.jpg)
Rheumatoid arthritis synovial fluid neutrophils drive inflammation through production of chemokines, reactive oxygen species and neutrophil extracellular traps
Cedrol attenuates collagen-induced arthritis in mice and modulates the inflammatory response in LPS-mediated fibroblast-like synoviocytes - Food & Function (RSC Publishing)
![Exploring the role of monocyte chemoattractant protein-1 in fibroblast-like synovial cells in rheumatoid arthritis [PeerJ]](https://ars.els-cdn.com/content/image/1-s2.0-S0753332222002359-gr1.jpg)
The enhanced mitochondrial dysfunction by cantleyoside confines inflammatory response and promotes apoptosis of human HFLS-RA cell line via AMPK/Sirt 1/NF-κB pathway activation - ScienceDirect
![Exploring the role of monocyte chemoattractant protein-1 in fibroblast-like synovial cells in rheumatoid arthritis [PeerJ]](https://dfzljdn9uc3pi.cloudfront.net/2021/11973/1/fig-2-full.png)
Exploring the role of monocyte chemoattractant protein-1 in fibroblast-like synovial cells in rheumatoid arthritis [PeerJ]
![Exploring the role of monocyte chemoattractant protein-1 in fibroblast-like synovial cells in rheumatoid arthritis [PeerJ]](https://www.frontiersin.org/files/Articles/655477/fimmu-12-655477-HTML/image_m/fimmu-12-655477-g001.jpg)
Frontiers A Tale of Two Immune Cells in Rheumatoid Arthritis: The Crosstalk Between Macrophages and T Cells in the Synovium
![Exploring the role of monocyte chemoattractant protein-1 in fibroblast-like synovial cells in rheumatoid arthritis [PeerJ]](https://ars.els-cdn.com/content/image/1-s2.0-S0006295221004317-gr1.jpg)
New potential therapeutic approaches targeting synovial fibroblasts in rheumatoid arthritis - ScienceDirect
![Exploring the role of monocyte chemoattractant protein-1 in fibroblast-like synovial cells in rheumatoid arthritis [PeerJ]](https://dfzljdn9uc3pi.cloudfront.net/2021/11973/1/fig-10-full.png)
Exploring the role of monocyte chemoattractant protein-1 in fibroblast-like synovial cells in rheumatoid arthritis [PeerJ]
![Exploring the role of monocyte chemoattractant protein-1 in fibroblast-like synovial cells in rheumatoid arthritis [PeerJ]](https://media.springernature.com/full/springer-static/image/art%3A10.1038%2Fnrrheum.2014.80/MediaObjects/41584_2014_Article_BFnrrheum201480_Fig1_HTML.jpg)
The multifactorial role of neutrophils in rheumatoid arthritis
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