Metabolic engineering of Deinococcus radiodurans for pinene

Background The objective of this work was to engineer Deinococcus radiodurans R1 as a microbial cell factory for the production of pinene, a monoterpene molecule prominently used for the production of fragrances, pharmaceutical products, and jet engine biofuels. Our objective was to produce pinene from glycerol, an abundant by-product of various industries. Results To enable pinene production in D. radiodurans, we expressed the pinene synthase from Abies grandis, the geranyl pyrophosphate (GPP) synthase from Escherichia coli, and overexpressed the native 1-deoxy-d-xylulose 5-phosphate synthase. Further, we disrupted the deinoxanthin pathway competing for the substrate GPP by either inactivating the gene dr0862, encoding phytoene synthase, or substituting the native GPP synthase with that of E. coli. These manipulations resulted in a D. radiodurans strain capable of producing 3.2 ± 0.2 mg/L pinene in a minimal medium supplemented with glycerol, with a yield of 0.13 ± 0.04 mg/g glycerol in shake flask cultures. Additionally, our results indicated a higher tolerance of D. radiodurans towards pinene as compared to E. coli. Conclusions In this study, we successfully engineered the extremophile bacterium D. radiodurans to produce pinene. This is the first study demonstrating the use of D. radiodurans as a cell factory for the production of terpenoid molecules. Besides, its high resistance to pinene makes D. radiodurans a suitable host for further engineering efforts to increase pinene titer as well as a candidate for the production of the other terpenoid molecules.

Metabolic engineering of Deinococcus radiodurans for pinene production from glycerol, Microbial Cell Factories

The schematic of the terpene synthetic pathway and the functional

PDF] Metabolic Engineering of Deinococcus radiodurans for the Production of Phytoene.

Loop Carsten Jers

Accumulation of Mn(II) in Deinococcus radiodurans Facilitates Gamma-Radiation Resistance

Microbial synthesis of pinene.

Microbial Cell Factories

Toward improved terpenoids biosynthesis: strategies to enhance the capabilities of cell factories, Bioresources and Bioprocessing

Microbial Cell Factories

Engineering Robustness of Microbial Cell Factories - Gong - 2017 - Biotechnology Journal - Wiley Online Library

Poster Session

Week 1 Discussion Micro.docx - Microbiology is both a basic and an applied science. It is not a single subject! It has many areas of specialization