IJMS, Free Full-Text
Por um escritor misterioso
Last updated 25 março 2025

The human Transient Receptor Potential A1 (hTRPA1) ion channel, also known as the wasabi receptor, acts as a biosensor of various potentially harmful stimuli. It is activated by a wide range of chemicals, including the electrophilic compound N-methylmaleimide (NMM), but the mechanism of activation is not fully understood. Here, we used mass spectrometry to map and quantify the covalent labeling in hTRPA1 at three different concentrations of NMM. A functional truncated version of hTRPA1 (Δ1-688 hTRPA1), lacking the large N-terminal ankyrin repeat domain (ARD), was also assessed in the same way. In the full length hTRPA1, the labeling of different cysteines ranged from nil up to 95% already at the lowest concentration of NMM, suggesting large differences in reactivity of the thiols. Most important, the labeling of some cysteine residues increased while others decreased with the concentration of NMM, both in the full length and the truncated protein. These findings indicate a conformational switch of the proteins, possibly associated with activation or desensitization of the ion channel. In addition, several lysines in the transmembrane domain and the proximal N-terminal region were labeled by NMM, raising the possibility that lysines are also key targets for electrophilic activation of hTRPA1.

IJMS, Free Full-Text

IJMS, Free Full-Text

Ijms Free Full Text Neural And Hormonal Basis Of 49680

IJMS, Free Full-Text
Fair Priced FavoriteIJMS, Free Full-Text, louis velasquez cgm

IJMS, Free Full-Text

IJMS, Free Full-Text

IJMS, Free Full-Text

IJMS, Free Full-Text

IJMS, Free Full-Text
Recomendado para você
-
NMM!Sans skin update25 março 2025
-
Color online) Density of states of the iron atom. (a) NM-P4/nmm, (b)25 março 2025
-
Sports SVG Basketball Team Football File Sports School Vinyl - Inspire Uplift25 março 2025
-
N.M.M CREW25 março 2025
-
NMM-square-logo - Hammersmith Broadway25 março 2025
-
does RFS count as a flight sim here?(i couldn't afford better) : r/flightsim25 março 2025
-
EG534 EG535 NMM-B681 For Lenovo Ideapad 330-15ARR Laptop Motherboard With R3-2200 R5-2500 R7-2700 CPU R540 2G GPU 4GB RAM - AliExpress25 março 2025
-
How OWG Grew Its Azure Practice 10x and Found Power in Partnership with Nerdio - Nerdio25 março 2025
-
New Mexico Medical Insurance Pool When no one else will help, we're here.25 março 2025
-
Home - News Mag Media25 março 2025
você pode gostar
-
adesivo de uma máscara de caveira assustadora de desenho animado 11502721 Vetor no Vecteezy25 março 2025
-
Assistir Mamahaha no Tsurego ga Motokano datta - Episódio - 225 março 2025
-
Onde posso verificar o resultado dos jogos lotéricos online? Confira - João Financeira25 março 2025
-
ip grabber discord|TikTok Search25 março 2025
-
Live Wire (Live at the Old Waldorf, San Francisco, Ca, 197725 março 2025
-
ORTHODOX CHRISTIANITY THEN AND NOW: The Experience of an American25 março 2025
-
Dollar Rate in Pakistan: USD to PKR Rate Today 23 August 202225 março 2025
-
Wendel Bezerra não me processa por favor 😅😅😅😅#WendelBezerra25 março 2025
-
gamepass for jailbreak - Roblox25 março 2025
-
PlayStation Plus unveils monthly games for November25 março 2025