Advanced Quantitative Fluorescence Microscopy to Probe the Molecular Dynamics of Viral Entry, Science Lab

Viral entry into the host cell requires the coordination of many cellular and viral proteins in a precise order. Modern microscopy techniques are now allowing researchers to investigate these interactions with higher spatiotemporal resolution than ever before. Here we present two examples from the field of HIV research that make use of an innovative quantitative imaging approach as well as cutting edge fluorescence lifetime-based confocal microscopy methods to gain novel insights into how HIV fuses to cell membranes and enters the cell.

Mucins Inhibit Coronavirus Infection in a Glycan-Dependent Manner

Measurement Assurance for Quantitative Cell Imaging by Optical Microscopy

Quantum dot as probe for disease diagnosis and monitoring - Mukherjee - 2016 - Biotechnology Journal - Wiley Online Library

Primate-conserved carbonic anhydrase IV and murine-restricted LY6C1 enable blood-brain barrier crossing by engineered viral vectors

Optimized production and fluorescent labeling of SARS-CoV-2 virus-like particles

Single-molecule localization microscopy

A spatial multi-scale fluorescence microscopy toolbox discloses entry checkpoints of SARS-CoV-2 variants in Vero E6 cells - Computational and Structural Biotechnology Journal

Quantitative four-dimensional tracking of cytoplasmic and nuclear HIV-1 complexes

Nonclinical pharmacokinetics and biodistribution of VSV-GP using methods to decouple input drug disposition and viral replication: Molecular Therapy - Methods & Clinical Development

Peptide-Induced Fusion of Dynamic Membrane Nanodomains: Implications in a Viral Entry

Advanced Quantitative Fluorescence Microscopy to Probe the Molecular Dynamics of Viral Entry, Science Lab

Altered host protease determinants for SARS-CoV-2 Omicron

Macrophage Sult2b1 promotes pathological neovascularization in age-related macular degeneration