Expediting Antibody Discovery with a Cell and Bead Multiplexed
Por um escritor misterioso
Last updated 20 abril 2025

A high-throughput, multiplexed cell and bead-based competition assay that identifies and quantitates mouse IgG isotypes and assesses cell health is developed that can reduce the time to antibody cloning and production. With advances in molecular engineering and humanization, monoclonal antibodies are one of the fastest-growing classes of biopharmaceuticals. During antibody discovery, antibody from hybridoma or primary B-cell supernatants is screened for the desired binding characteristics, and secondary screens measure antibody function and concentration, identify immunoglobulin G (IgG) isotype, and assess cell health. In order to expedite the antibody discovery process, we developed a high-throughput, multiplexed cell and bead-based competition assay that identifies and quantitates mouse IgG isotypes and assesses cell health. No differences in assay performance were observed between single and multiplex formats. The linear range of the assay was from 0.5 to 50 µg/mL, and washing was not required, decreasing assay time and variability. Slight modifications to the protocol allowed quantification of dilute antibody supernatants (0.1–5 µg/mL). Using hybridoma cultures, we showed that cell viability measurements in the assay did not interfere with the bead-based IgG measurements. The assay described here is a simple mix-and-read, no-dilution screen that can reduce the time to antibody cloning and production. The high-content data can differentiate monoclonal and polyclonal wells, determine IgG quantity for downstream functional assays, provide isotype information, and monitor cell proliferation and viability.

A Scalable Pipeline for High-Throughput Flow Cytometry - SLAS

B cell Sorting Capabilities

CiteAb introductory guides: Multiplexed antibody based imaging

Multiplex Cytokine Immunoassays

Using Multiplexed Screening for Advanced Therapeutic Antibody

Multiplexed Immunoassays: Small Samples, Big Pictures

Characterization of antibodies from the established hybridomas by

ELISA in the multiplex era: Potentials and pitfalls - Tighe - 2015

Dynamic Shifts in Precision Medicine

Biopharma Science How To Guides, Page 3
Recomendado para você
-
Omega Legends - Apps on Google Play20 abril 2025
-
PDF) Streptococcus pyogenes evades adaptive immunity through20 abril 2025
-
Systemic IgG repertoire as a biomarker for translocating gut20 abril 2025
-
Frontiers Unraveling cross-reactivity of anti-glycan IgG20 abril 2025
-
Michael Ramova (@ramova) / X20 abril 2025
-
PDF) Fc-Mediated Functions of Porcine IgG Subclasses20 abril 2025
-
Reinvestigating the Coughing Rat Model of Pertussis To Understand20 abril 2025
-
Nfkb2 deficiency and its impact on plasma cells and immunoglobulin20 abril 2025
-
The G2 phase controls binary division of Toxoplasma gondii20 abril 2025
-
Azerbaijani Special forces by GhostDesign1 on DeviantArt20 abril 2025
você pode gostar
-
Mari's Emotion Chart from OMORI | Sticker20 abril 2025
-
GitHub - ttkienn/KaguyaExploit: Exploit Roblox Using WeAreDevs Api20 abril 2025
-
All the Warriors Super Edition Books in Order20 abril 2025
-
Guzzlord (SV26/SV94) [Sun & Moon: Hidden Fates - Shiny Vault]20 abril 2025
-
Raw do Cap final de DanMachi - Dungeon ni Deai Brasil20 abril 2025
-
Universal hinting at name of new theme park.20 abril 2025
-
Steam Community :: Guide :: How to make Fallout 3 GOTY work on20 abril 2025
-
SOLVED] - Rockstar Launcher crashes when it tries to connect to20 abril 2025
-
Extreme ironing' probably won't replace NFL20 abril 2025
-
Como Jogar RPG Na Quarentena?20 abril 2025