Biology, Free Full-Text
Por um escritor misterioso
Last updated 01 junho 2024
Microbial symbionts range from mutualistic to commensal to antagonistic. While these roles are distinct in their outcome, they are also fluid in a changing environment. Here, we used the Lotus japonicus–Mesorhizobium japonicum symbiosis to investigate short-term and long-term shifts in population abundance using an effective, fast, and low-cost tracking methodology for M. japonicum. We use quantitative polymerase chain reaction (qPCR) to track previously generated signature-tagged M. japonicum mutants targeting the Tn5 transposon insertion and the flanking gene. We used a highly beneficial wild type and moderately beneficial and non-beneficial mutants of M. japonicum sp. nov. to demonstrate the specificity of these primers to estimate the relative abundance of each genotype within individual nodules and after serial transfers to new hosts. For the moderate and non-beneficial genotypes, qPCR allowed us to differentiate genotypes that are phenotypically indistinguishable and investigate host control with suboptimal symbionts. We consistently found the wild type increasing in the proportion of the population, but our data suggest a potential reproductive trade-off between the moderate and non-beneficial genotypes. The multi-generation framework we used, coupled with qPCR, can easily be scaled up to track dozens of M. japonicum mutants simultaneously. Moreover, these mutants can be used to explore M. japonicum genotype abundance in the presence of a complex soil community.
Free-text inspiration search for systematic bio-inspiration
PDF) Ophidiomyces ophidiicola in free-ranging and captive snakes
The Quarterly Review of Biology
Text Book of Biology, Part 1: Vertebrata by H. G. Wells
Biology and Life Sciences Forum, Free Full-Text
Premium Vector Biology 3d text effect vector download
This book is for your convenience. It is available for printing from our site. This book contains the sheets you would print if using the online
EP Biology Printables: Levels 1-4: Part of the Easy Peasy All-in-One Homeschool
Educart NEET BIOLOGY 35 Years Chapterwise Topicwise Solved
IJMS, Free Full-Text
Biology, Free Full-Text
Animals, Free Full-Text
Skmer: assembly-free and alignment-free sample identification
Free Radical Biology and Medicine, Journal
Recomendado para você
-
Bronstein01 junho 2024
-
COMEDIAN - Alex Edelman - The Hampton Synagogue01 junho 2024
-
Como chegar até Bronstein Medicina Diagnóstica - Méier de Ônibus ou Trem?01 junho 2024
-
Como chegar até Bronstein Madureira (Laboratorio Bronstein01 junho 2024
-
Bastian RIECK, Principal Investigator, Ph.D.01 junho 2024
-
Mitogenome-wise codon usage pattern from comparative analysis of01 junho 2024
-
Student Scientists Shine at Elgin School Science Fair - Patagonia01 junho 2024
-
Ultrafast Exciton Self-Trapping upon Geometry Deformation in Perylene-Based Molecular Aggregates01 junho 2024
-
Multi-temporal ecological niche modeling for bird conservation in the face of climate change scenarios in Caatinga, Brazil [PeerJ]01 junho 2024
-
A Low-Level Active Vision Framework for Collaborative Unmanned Aircraft Systems01 junho 2024
você pode gostar
-
CapCut_mê kamado nezuko01 junho 2024
-
GTA 5 Mods Mono in Little Nightmares 2 - GTA 5 Mods Website01 junho 2024
-
I'am a sword user why? (Before the phoenix i got light) : r01 junho 2024
-
Hooded Puffer Jacket - Black - Ladies01 junho 2024
-
Onde assistir ao vivo Portuguesa x Desportivo Brasil pela Copa01 junho 2024
-
Peter Grill and the Philosopher's Time: Super Extra01 junho 2024
-
Jogo do CD Uai Urquiza (Feminino) hoje ⚽ CD Uai Urquiza (Feminino01 junho 2024
-
Guide For Untitled Goose Game Free 2021 APK voor Android Download01 junho 2024
-
Sonic the Hedgehog 2 - VGDB - Vídeo Game Data Base01 junho 2024
-
1990 Tour de France - Wikipedia01 junho 2024